Value of detecting bacterial 16S and 23S rRNA in interface membrane in diagnosis of periprosthetic joint infection / 南方医科大学学报
Journal of Southern Medical University
;
(12): 190-194, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-232486
ABSTRACT
<p><b>OBJECTIVE</b>To explore the value of detecting bacterial 16S rRNA with 23S rRNA in the diagnosis of periprosthetic joint infection (PJI).</p><p><b>METHODS</b>A prospective study was conducted among 67 patients with previous total hip arthroplasty (THA) undergoing a reoperation for infection (23 patients) or aseptic loosening (44 patients). Bacterial 16S rRNA and 23S rRNA in the interface membrane were detected by real-time PCR and their value in diagnosis of PJI was assessed.</p><p><b>RESULTS</b>The 16S rRNA and 23S rRNA showed no significant difference in their power in the diagnosis of PJI. The detection of 16S rRNA/23S rRNA showed a higher sensitivity and a greater negative predictive value in PJI diagnosis than the detection of 16S rRNA+23S rRNA (95.7% vs 52.2%, P<0.01; 97.6% vs 79.6%, P=0.01). The specificity, positive predictive value, and accuracy of the 4 diagnostic strategies were not significantly different.</p><p><b>CONCLUSIONS</b>The diagnostic power of 16S rRNA and 23S rRNA was similar in detecting PJI. Compared with the diagnostic strategy with 16S rRNA+23S rRNA, 16S rRNA/23S rRNA is more sensitive in detecting PJI.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reoperation
/
RNA, Bacterial
/
RNA, Ribosomal, 16S
/
RNA, Ribosomal, 23S
/
Arthritis, Infectious
/
Prospective Studies
/
Sensitivity and Specificity
/
Prosthesis-Related Infections
/
Arthroplasty, Replacement, Hip
/
Diagnosis
Type of study:
Diagnostic study
/
Observational study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article
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