Tal1 promotes proliferation of acute lymphoblastic leukemia Jurkat cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 78-82, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-232507
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of Tal1 gene, which is aberrantly expressed in 40%-60% of patients with T lymphocytic leukemia (T-ALL), in the proliferation of T-ALL cells.</p><p><b>METHODS</b>We established stable Jurkat-siTal1 and Jurkat-T1 cell lines by trasnfecting T-ALL Jurkat cells with lentiviral vectors to knock-down or overexpress Tal1. Jurkat cells transfected with negative control siRNAs for Tal1 knock-down (Jurkat-mock1) and over-expression(Jurkat-mock2) served as the control cells. The proliferation of the cells lines was assessed using CCK-8 assay, and the cell cycle distribution was determined by flow cytometry. The mRNA and protein expressions of cyclin-dependent kinase inhibitor 2 (CDKN2A) and cyclin-dependent kinase inhibitor 1 (CDKN2B) were measured by real-time RT-PCR and Western blotting, respectively.</p><p><b>RESULTS</b>Jurkat-T1 cells showed more active proliferation in vitro than Jurkat-mock2 cells, while Jurkat-siTal1 cells showed slower growth than Jurkat-mock1 cells. In Jurkat-T1 cells, G0/G1 phase cells were decreased and S phase cells increased compared with Jurkat-mock2 cells, and Jurkat-siTal1 cells showed increased G0/G1 phase cells and decreased S phase cells compared with Jurkat-mock1 cells. Real-time RT-PCR and Western blotting showed that Tal1 inhibited the cellular expression of CDKN2A and CDKN2B at both mRNA and protein levels.</p><p><b>CONCLUSION</b>Tal1 promotes the growth and the transition from G0/G1 phase to S phase in T-ALL cells Jurkat by inhibiting the expressions of G0/G1 and S phase negative regulatory proteins CDKN2A and CDKN2B.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Cell Cycle
/
Proto-Oncogene Proteins
/
Apoptosis
/
Lentivirus
/
Jurkat Cells
/
Cyclin-Dependent Kinase Inhibitor p16
/
RNA, Small Interfering
/
Cell Proliferation
/
Cyclin-Dependent Kinase Inhibitor p15
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article
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