Effect of Astragalus mongholicus polysaccharides on gene expression profiles of dendritic cells isolated from healthy donors / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1802-1805, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-232523
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-atherosclerosis mechanism of Astragalus mongholicus polysaccharides (APS) by examining its effect on gene expression profiles of the dendritic cells (DCs) from healthy donors.</p><p><b>METHODS</b>Peripheral blood DCs from healthy donors were incubated with 200 mg/L APS overnight, and changes in the gene expression profiles were investigated using microarray technique and RT-PCR.</p><p><b>RESULTS</b>Compared with the control cells, APS-treated DCs showed significantly up-regulated expressions of CD36 (0.97 ± 0.23 vs 5.45 ± 1.14) and IL-27 (1.08 ± 0.22 vs 2.97 ± 0.61) and down-regulated expression of expression of IFI16 (0.98 ± 0.18 vs 0.46 ± 0.11).</p><p><b>CONCLUSIONS</b>APS can promote the maturation and differentiation of DCs by up-regulating CD36 and IL-27 and down-regulating IFI16, and thus positively affects the occurrence and progression of the atherosclerosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphoproteins
/
Polysaccharides
/
Dendritic Cells
/
Nuclear Proteins
/
Cell Differentiation
/
Chemistry
/
Interleukins
/
CD36 Antigens
/
Astragalus Plant
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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