Combined detection of thyroid transcription factor 1 and napsin A in pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1610-1613, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-232561
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the value of detecting thyroid transcription factor 1 (TTF-1) and Noval aspartic proteinase of pepsin family A (napsin A) in pleural fluid cell blocks in cytopathologic diagnosis of pulmonary adenocarcinoma.</p><p><b>METHODS</b>Conventional cell smears of pleural effusions were obtained from 48 patients with a history of lung adenocarcinoma for cytological analysis. The cell blocks were prepared using the cytological specimens and examined with immunohistochemistry for TTF-1 and napsin A. The rates of a positive diagnosis of pulmonary adenocarcinoma were compared between the two methods, and the diagnositic value of TTF-1 and napsin A in pleural fluid cell blocks was evaluated.</p><p><b>RESULTS</b>Immuno- histochemistry of the cell block sections yielded a significantly higher positive rate of diagnosis than cytological analysis of conventional cell smear (84.44% vs 55.56%, P<0.05). Most of the pleural fluid cell blocks showed positive expressions of TTF-1 (36/38, 94.74%) and napsin A (30/38, 78.95%), and none of samples showed TTF-1 or napsin A expression in the mesothelial cells (P<0.05). The combination detection of TTF-1 and napsin A in pleural fluid cell blocks had a high diagnosis value with a diagnostic sensitivity of 97.37% and a specificity of 100% for pulmonary adenocarcinoma.</p><p><b>CONCLUSIONS</b>The combined detection of TTF-1 and napsin A in pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pleural Effusion
/
Transcription Factors
/
Immunohistochemistry
/
Nuclear Proteins
/
Adenocarcinoma
/
Biomarkers, Tumor
/
Aspartic Acid Endopeptidases
/
Sensitivity and Specificity
/
Diagnosis
/
Thyroid Nuclear Factor 1
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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