Optimization on the production of analgesic peptide from Buthus martensii Karsch in Pichia pastoris / 药学学报
Acta Pharmaceutica Sinica
;
(12): 91-94, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-232591
ABSTRACT
The technology of liquid fermentation for producing the recombinant analgesic peptide BmK AngM1 from Buthus martensii Karsch in Pichia pastoris was studied by single-factor and orthogonal test. The results showed that the optimal culture conditions were as follows 1.2% methanol, 0.6% casamino acids, initial pH 6.0, and three times of basal inoculation volume. Under the above culture conditions, the expression level of recombinant BmK AngM1 in Pichia pastoris was above 500 mg x L(-1), which was more than three times of the control. The study has laid a foundation for the large-scale preparation of BmK AngM1 to meet the needs of theoretical research of BmK AngM1 and development of new medicines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Pharmacology
/
Pichia
/
Scorpion Venoms
/
Scorpions
/
Recombinant Proteins
/
Gene Expression
/
Chemistry
/
Methanol
/
Fermentation
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2009
Type:
Article
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