Isolation and expression profiling of transformer 2 gene in Aedes aegypti / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1583-1589, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-232746
ABSTRACT
<p><b>OBJECTIVE</b>To isolate, identify and analyze the sex-determining gene Transformer 2 (Aaetra2) of the major vector mosquito Aedes aegypti.</p><p><b>METHODS</b>tBLASTn program, RT-PCR and RACE methods were used to obtain the full-length cDNA of Aaetra2. Multiple alignments of nucleotide and amino acid sequences were conducted, and the different domains in tra2 protein were indentified. RT-PCR of the total RNA extracted from different tissue from the mosquitoes in different developmental stages was performed using specific primers.</p><p><b>RESULTS</b>Two genes, namely Aaetra2-α and Aaetra2-β, were identified in different supercontig locations. The multi-transcripts were expressed by means of alternative promoters or terminators. The different domains in tra2 protein were defined as RS-rich N-terminal region, RNA recognition motif-RRM, linker region, and RS-rich C-terminal region. Both Aaetra2-α and Aaetra2-β showed sustained expression throughout the developmental stages of Ae.aegypti, and in all the tissues without a sex specificity.</p><p><b>CONCLUSION</b>Aaetra2 gene has multiple isoforms and is mapped to multiple locations in the genome. Aaetra2 has conservative functional domains of the sex-determining gene tra2. For Ae.agypti, Aaetra2 shows the potential as a new target for release of insects carrying a dominant lethal (RIDL) technology based on transgenic mosquitoes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Ribonucleoproteins
/
Sex Differentiation
/
Sequence Alignment
/
Amino Acid Sequence
/
RNA-Binding Proteins
/
Genes, Insect
/
Gene Expression Regulation, Developmental
/
Insect Proteins
/
Aedes
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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