Pulmonary expression of Nr1d1 in rats with ventilation-induced lung injury / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the changes in the expression of nuclear receptor subfamily1 (Nr1d1) in the lungs of rats with ventilation-induced lung injury and explore the molecular mechanism.</p><p><b>METHODS</b>Seventy-two rats were randomly divided into 4 groups for observation at 4 time points (100 AM, 700 AM, 100 PM and 700 PM), and each group was further divided into 3 subgroups (Group FB, Group LV and Group HV). After ventilation for 3 h, the rats were sacrificed and lung tissue samples were obtained for testing the expression of Nr1d1 using gene chip technology and RT-PCR.</p><p><b>RESULTS</b>Nr1d1 gene expression was down-regulated in HV group as shown by gene chip RT-PCR data. Cosine analysis showed that Nr1d1 expression exhibited a rhythmicity in FB group (P<0.05).</p><p><b>CONCLUSION</b>The rhythmicity of Nr1d1 expression disappears as the tidal volume the increase. Diminished Nr1d1 expression is closely correlated with ventilation-induced lung injury in rats.</p>