Effects of peroxisome proliferator-activated receptors γ on the expression of insulin receptor substrate-4 gene in rat cortical neurons and mouse brain / 南方医科大学学报
Journal of Southern Medical University
; (12): 1463-1466, 2013.
Article
in Zh
| WPRIM
| ID: wpr-232774
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on insulin receptor substrate-4 (IRS-4) gene expression in the brain.</p><p><b>METHODS</b>Primarily cultured cortical neurons from E17-18 Sprague Dawley rats, after 1 week of plating, were exposed to 10 µmol/L PPARγ agonist rosiglitazone for 0, 1, 4 or 24 h. Adult C57BL/6J mice or conditional brain PPARγ knock-out mice (B-PPARγ-KO, BKO) received an intraperitoneal injection of rosiglitazone in 10% DMSO at 12 mg/kg or injection of the same volume of saline containing 10% DMSO. The effect of rosiglitazone on the survival of the neurons was examined by MTT assay. The expression of IRS-4 mRNA was analyzed by real-time quantitative PCR.</p><p><b>RESULTS</b>The survival of the cortical neurons showed no significant difference between the agonist groups and the control group. The expression of IRS-4 mRNA was significantly up-regulated in the cortical tissues and neurons of the agonist groups compared with the control groups (P<0.05), but in BKO mice without treatment, IRS-4 mRNA expression was significantly down-regulated (P<0.05).</p><p><b>CONCLUSION</b>PPARγ can enhance the expression of IRS-4 mRNA in the brain.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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RNA, Messenger
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Up-Regulation
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Cell Survival
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Cells, Cultured
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Cerebral Cortex
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Rats, Sprague-Dawley
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Mice, Knockout
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Gene Transfer Techniques
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Cell Biology
Limits:
Animals
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Pregnancy
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article