Identification of the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1374-1379, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-232980
ABSTRACT
Sinisan is a widely used traditional Chinese medicine (TCM) in treating various diseases; however, the in vivo metabolic profile of its multiple components remains unknown. In this paper, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was applied to identify the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration. Using MS(E) and mass defect filter techniques, 41 metabolites of 10 parent compounds (naringin, naringenin, hesperidin, neohesperidin, liquiritin, liquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, saikosaponin a and saikosaponin d) were detected and tentatively identified. It was shown by our results that these compounds was metabolized to the forms of hydroxylation, glucuronidation, sulfation, glucuronidation with sulfation and glucuronidation with hydroxylation in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Drugs, Chinese Herbal
/
Pharmacokinetics
/
Random Allocation
/
Chemistry
/
Administration, Oral
/
Chromatography, High Pressure Liquid
/
Rats, Sprague-Dawley
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Glycyrrhizic Acid
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS