Anti-MDR tumor mechanism of CIP-36, a podophyllotoxin derivative / 药学学报
Acta Pharmaceutica Sinica
; (12): 1193-1198, 2011.
Article
in Zh
| WPRIM
| ID: wpr-233013
Responsible library:
WPRO
ABSTRACT
This study is to investigate the antitumor activity of CIP-36 on multidrug resistant human oral squamous carcinoma cell line (KBV200 cells) in vitro and the possible anticancer mechanisms. MTT assay, Hoechst fluorescein stain, RT-PCR and immunohistochemistry were carried out on KBV200 and KB cells. The growth of many tumor cells was obviously inhibited by CIP-36, especially the multidrug resistant cells KBV200. Obvious apoptosis could be observed in the Hoechst 33342 staining experiments. The results of RT-PCR showed that the levels of p53, p21, caspase-3 and bax mRNA increased, and meanwhile the expression of mdr-1 and bcl-2 mRNA decreased in a dose-dependent manner. The data were significantly different from that of vehicle. The expression of P-gp significantly decreased with the increasing dosage of CIP-36 examined by immunohistochemistry. It can be concluded that CIP-36 could change resistance-related genes and proteins to overcome multidrug resistance in the KBV200 cell line.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Podophyllotoxin
/
RNA, Messenger
/
Mouth Neoplasms
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KB Cells
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Carcinoma, Squamous Cell
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Tumor Suppressor Protein p53
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Proto-Oncogene Proteins p21(ras)
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Apoptosis
Limits:
Humans
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Type:
Article