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Development of fluorescence imaging based assay for screening compounds with anti-migration activity / 药学学报
Acta Pharmaceutica Sinica ; (12): 793-797, 2011.
Article in Chinese | WPRIM | ID: wpr-233054
ABSTRACT
In the present study, A fluorescent imaging-based high-throughput screening method was developed for identifying anti-migratory compounds with 96-well Transwell plates. The correlation, precision and stability of this method were examined and the incubation time of dye Hoechst 33342 in addition to migration time was optimized. In addition, The inhibitory activity of anti-cancer drug paclitaxel on tumor cell migration was assayed and an IC50 value of 0.717 micromol x L(-1) was obtained. Using this method, 24 components from Rhizoma Alismatis were screened and one component with anti-migration activity was found. These results show that the new proposed method with good precision, stability and linear range has the potential to assay the inhibitory activity of anticancer compounds.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plants, Medicinal / Drug Screening Assays, Antitumor / Drugs, Chinese Herbal / Cell Movement / Chemistry / Paclitaxel / Inhibitory Concentration 50 / Rhizome / Alisma Type of study: Diagnostic study / Screening study Limits: Humans Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plants, Medicinal / Drug Screening Assays, Antitumor / Drugs, Chinese Herbal / Cell Movement / Chemistry / Paclitaxel / Inhibitory Concentration 50 / Rhizome / Alisma Type of study: Diagnostic study / Screening study Limits: Humans Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2011 Type: Article