Anti-tumor efficacy of P53 with 9R cell-penetrating peptides / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 955-964, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-233184
ABSTRACT
To enhance the penetration of P53 into tumor cells by fusion it with the cell penetrating peptide 9R. The fusion gene of 9R-p53 was cloned into the expression vector. The fusion protein, CPPs-P53, was expressed and purified. We detected the rate of cell growth inhibition and apoptosis by MTT and Annexin-V-FITC/PI double stained method respectively for measuring its effect on tumor cells. CPPs-P53 and P53 were successfully expressed and purified, the purity of both proteins reached up to 90%. MTT assay showed that the cell growth inhibition by CPPs-P53 was more efficient than P53, and the rate of cell growth inhibition is dose-dependent. The apoptosis experiment showed that P53 could induce apoptosis of tumor cells. Compared with the P53, CPPs-P53 had a more significant effect in inducing cell apoptosis (**P < 0.01). The CPPs-P53 shows more significant effects than P53 in inhibiting cell growth and inducing apoptosis on tumor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Tumor Suppressor Protein p53
/
Apoptosis
/
Cell Line, Tumor
/
Cell-Penetrating Peptides
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
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