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DNA prime followed by protein boost enhances the protective efficacy against Schistosoma japonicum infection in mice / 生物工程学报
Chinese Journal of Biotechnology ; (12): 814-822, 2013.
Article in Chinese | WPRIM | ID: wpr-233198
ABSTRACT
Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China. Vaccination is needed as a complementary approach to the ongoing control programs. In the present study, we determined if the efficacies of DNA vaccine encoding the SjGST and Sj32 asparaginyl endopeptidase protein could be enhanced by boosting with SjGST-32 protein vaccines. Mice were inoculated with a VR1012-SjGST-32 DNA vaccine followed by boosting with rSjGST-32 at 0, 14 and 28 d. Two weeks after the final boost, mice were challenged percutaneously with cercariae. On day 45 following the challenge, all mice were sacrificed and the numbers of recovered worms and hepatic eggs were counted. Moreover, we analyzed the immune response among various vaccination groups. The results showed that DNA vaccine efficacy was enhanced when mice were boosted with protein vaccine. Adult worm and liver egg burdens were reduced 42.3% and 59.6%, respectively. We further found that DNA vaccine followed by boosting with protein significantly increased the IgG titer and T cell proliferation over those seen in mice vaccinated solely with DNA vaccines. Furthermore, the higher level of IFN-gamma expression in the splenetic CD4+ T cell showed that DNA prime-Protein boosting vaccine induced CD4+ Th1-type responses. Thus, DNA vaccine efficacy was significantly enhanced via boosting protein vaccine which might provide a basis for rational application of the Schistosoma vaccine.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Schistosoma japonicum / Recombinant Fusion Proteins / Schistosomiasis japonica / Helminth Proteins / Immunization, Secondary / Vaccination / Vaccines, DNA / Allergy and Immunology / Glutathione Transferase / Methods Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Schistosoma japonicum / Recombinant Fusion Proteins / Schistosomiasis japonica / Helminth Proteins / Immunization, Secondary / Vaccination / Vaccines, DNA / Allergy and Immunology / Glutathione Transferase / Methods Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article