Cost-effective production of protein by using cellulose-binding domain fusion tag in Corynebacterium glutamicum / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 691-694, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-233208
ABSTRACT
The CBD gene from Trichoderma reesei was cloned into the Corynebacterium glutamicum secretion expression vector pXMJ19-sp, in which green fluorescent protein was inserted to obtain pXMJ19-sp-GFP-CBD. After induced by 0.5 mmol/L IPTG, GFP-CBD was expressed in Corynebacterium glutamicum at high level of 200 mg/L. The GFP-CBD could be purified to high purity with cellulose column. The results indicated CBD can be successfully used in Corynebacterium glutamicum expression system and thus offer an extremely simple, effective and scalable way for production of recombinant proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trichoderma
/
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Protein Engineering
/
Cellulose
/
Chemistry
/
Cloning, Molecular
/
Cost-Benefit Analysis
/
Cellulases
Type of study:
Health economic evaluation
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS