Key enzymes in butanol fermentation by a facultative anaerobe Bacillus sp. TSH1 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 620-629, 2013.
Article
in Zh
| WPRIM
| ID: wpr-233215
Responsible library:
WPRO
ABSTRACT
Bacillus sp. TSH1 is a butanol-producing microorganism newly isolated in our laboratory; it can grow and ferment under facultative anaerobic conditions, while sharing similar fermentation pathways and products with Clostridium acetobutylicum. To illustrate the relationships between the products and the enzyme activities in Bacillus sp. TSH1, key butanol- and ethanol-forming enzymes were studied, including butyraldehyde dehydrogenase, butanol dehydrogenase and alcohol dehydrogenase. The activities of the three enzymes increased rapidly after the initiation of fermentation. Activities of three enzymes peaked before 21 h, and simultaneously, product concentrations also began to increase gradually. The maximum activity of alcohol dehydrogenase was 0.054 U/mg at 12 h, butyraldehyde dehydrogenase 0.035 U/mg at 21 h and butanol dehydrogenase 0.055 U/mg at 15 h. The enzyme activities then decreased, but remained constant at a low level after 24 h, while the concentrations of butanol, acetone, and ethanol continued increasing until the end of the fermentation. The results will attribute to the understanding of the butanol metabolic mechanism, and provide a reference for further study of a facultative Bacillus metabolic pathway.
Full text:
1
Index:
WPRIM
Main subject:
Bacillus
/
Alcohol Dehydrogenase
/
Classification
/
Alcohol Oxidoreductases
/
Butanols
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Aldehyde Oxidoreductases
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Metabolic Networks and Pathways
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Fermentation
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Genetics
/
Anaerobiosis
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article