Molecular engineering of cellulase catalytic domain based on glycoside hydrolase family / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 422-433, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-233233
ABSTRACT
Molecular engineering of cellulases can improve enzymatic activity and efficiency. Recently, the Carbohydrate-Active enZYmes Database (CAZy), including glycoside hydrolase (GH) families, has been established with the development of Omics and structural measurement technologies. Molecular engineering based on GH families can obviously decrease the probing space of target sequences and structures, and increase the odds of experimental success. Besides, the study of cellulase active-site architecture paves the way toward the explanation of catalytic mechanism. This review focuses on the main GH families and the latest progresses in molecular engineering of catalytic domain. Based on the combination of analysis of a large amount of data in the same GH family and their conservative active-site architecture information, rational design will be an important direction for molecular engineering and promote the rapid development of the conversion of biomass.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Engineering
/
Cellulase
/
Chemistry
/
Evolution, Molecular
/
Directed Molecular Evolution
/
Catalytic Domain
/
Genetics
/
Glycoside Hydrolases
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS