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Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis / 生物工程学报
Chinese Journal of Biotechnology ; (12): 119-122, 2013.
Article in Chinese | WPRIM | ID: wpr-233262
ABSTRACT
Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Radiation Effects / Mutagenesis / Heavy Ions / Stramenopiles / Microalgae / Genetics / Lipids / Metabolism / Mutation Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Radiation Effects / Mutagenesis / Heavy Ions / Stramenopiles / Microalgae / Genetics / Lipids / Metabolism / Mutation Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article