Anti-oxidative and anti-inflammatory effects of Tagetes minuta essential oil in activated macrophages
Asian Pacific Journal of Tropical Biomedicine
;
(12): 219-227, 2014.
Article
in English
| WPRIM
| ID: wpr-233350
ABSTRACT
<p><b>OBJECTIVE</b>To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil.</p><p><b>METHODS</b>In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-oxidant capacity of T. minuta essential oil was examined by measuring reactive oxygen, reactive nitrogen species and hydrogen peroxide scavenging. The anti-inflammatory activity of T. minuta essential oil was determined through measuring NADH oxidase, inducible nitric oxide synthase and TNF-α mRNA expression in lipopolysacharide-stimulated murine macrophages using real-time PCR.</p><p><b>RESULTS</b>Gas chromatography-mass spectrometry analysis indicated that the main components in the T. minuta essential oil were dihydrotagetone (33.86%), E-ocimene (19.92%), tagetone (16.15%), cis-β-ocimene (7.94%), Z-ocimene (5.27%), limonene (3.1%) and epoxyocimene (2.03%). The T. minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC50 12-15 µg/mL, which indicated a potent radical scavenging activity. In addition, T. minuta essential oil significantly reduced NADH oxidase, inducible nitric oxide synthaseand TNF-α mRNA expression in the cells at concentrations of 50 µg/mL, indicating a capacity of this product to potentially modulate/diminish immune responses.</p><p><b>CONCLUSIONS</b>T. minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions.</p>
Full text:
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Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2014
Type:
Article
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