A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons / 대한해부학회지
Anatomy & Cell Biology
;
: 95-103, 2015.
Article
in English
| WPRIM
| ID: wpr-23347
ABSTRACT
We identified a neuroprotective single fraction among 62 ones of hexane extract from Uncaria sinensis (JGH43IA) and investigated its effects and mechanisms in primary cortical neurons. Pretreatment with JGH43IA showed a significantly increase cell viability in a dose-dependent manner with a decrease in the lactate dehydrogenase release. When we performed morphological assay and flow cytometry to determination of the type of cell death, pretreatment with JGH43IA showed a significant reduction of glutamate-induced apoptotic cell death. Then we explored the downstream signaling pathways of N-methyl-D-aspartate receptor (NMDAR) with calpain activation to elucidate possible pathways of neuroprotection by JGH43IA. Pretreatment with JGH43IA exhibited a significant attenuation of NMDAR GluN2B subunit activation and a decrease in active form of calpain 1 leading to subsequent cleavage of striatal-enriched protein tyrosine phosphatase (STEP). In addition, pretreatment with JGH43IA showed a marked increase of cAMP responsive element binding protein. These results suggest that JGH43IA may have neuroprotective effects through down-regulation of NMDAR GluN2B subunit and calpain 1 activation, and subsequent alleviation of STEP cleavage. This single fraction from U. sinensis might be a useful therapeutic agent for brain disorder associated with glutamate injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain Diseases
/
Calpain
/
Carrier Proteins
/
Down-Regulation
/
Cell Survival
/
N-Methylaspartate
/
Protein Tyrosine Phosphatases
/
Cell Death
/
Receptors, N-Methyl-D-Aspartate
/
Neuroprotective Agents
Language:
English
Journal:
Anatomy & Cell Biology
Year:
2015
Type:
Article
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