Effect of the NHE-1-specific inhibitor DMA on pHi, proliferation and apoptosis of HL-60/ADM cells in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 488-491, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-233561
ABSTRACT
The aim of this study was to evaluate the effect of dimethyl amiloride (DMA), a specific inhibitor of Na(+)/H(+) exchanger-1 (NHE-1), on intracellular pH value (pHi), proliferation and apoptosis of HL-60/ADM cells in vitro. After treatment with DMA at different doses, pHi of HL-60 and HL-60/ADM cell lines were determined by using pH-sensitive fluorescence dye BECEF-AM; the rate of growth inhibition of cells was detected with MTT assay; cell cycle was detected by flow cytometric DNA analysis; cell apoptosis was observed with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The results showed that pHi in HL-60/ADM cells was higher than that in HL-60 cells. After treatment with DMA at different doses, pHi decreased, the rate of growth inhibition and the rate of apoptotic cells in HL-60/ADM cells were all higher than those in HL-60 cells. Meanwhile, after treatment with DMA during 100 micromol/L to 150 micromol/L, the increase amplitude of G(0)/G(1) phase cells and the decrease amplitude of S + G(2)/M cells in HL-60/ADM cells were higher than those in HL-60 cells. It is concluded that by causing intracellular acidification, the NHE-1-specific inhibitor DMA inhibits proliferation of HL-60/ADM cells and induces apoptosis of HL-60/ADM cells, and the degree of this growth inhibition of HL-60/ADM cells is higher than that of HL-60 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Doxorubicin
/
Cell Cycle
/
Apoptosis
/
Sodium-Hydrogen Exchangers
/
HL-60 Cells
/
Drug Resistance, Neoplasm
/
Cell Proliferation
/
Amiloride
/
Hydrogen-Ion Concentration
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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