Expressions of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 in chronic myeloid leukemia cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 433-436, 2006.
Article
in Zh
| WPRIM
| ID: wpr-233574
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore the expression of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 in bcr/abl fusion gene positive CML cells, and to study the effects of P210(bcr/abl) fusion protein tyrosine kinase on expression of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 mRNAs in chronic myeloid leukemia cells. The expression levels of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 mRNA were detected by semi-quantitative RT-PCR in bcr/abl negative cells, bcr/abl positive cells, and P210(bcr/abl)-Rb-C-Box positive cells. The results showed that MIP-1alpha and CCR-1 mRNAs were expressed in bcr/abl negative cells, but not in positive cells. Both MCP-1 and CCR-2 mRNA cannot be detected in both bcr/abl positive and negative cells. After inhibiting P210(bcr/abl) tyrosine kinase activity by Rb-C-Box, expressions of MIP-1alpha and CCR-1 mRNAs were restored to normal (similar to P210(bcr/abl) negative cells). It is concluded that P210(bcr/abl) fusion protein inhibits the expression of MIP-1alpha and CCR-1 in chronic myeloid leukemia cells, but does not inhibit MCP-1 and CCR-2 mRNA expressions in these leukemia cells.
Full text:
1
Index:
WPRIM
Main subject:
Tumor Cells, Cultured
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Chemokine CCL2
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Macrophage Inflammatory Proteins
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Receptors, Chemokine
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Chemokine CCL3
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Chemokine CCL4
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Receptors, CCR1
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Receptors, CCR2
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Genetics
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article