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Regulation of hepatic stellate cell activation by interleukin-10/platelet derived growth factor/mitogen-activated protein kinase pathway / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 35-37, 2005.
Article in Chinese | WPRIM | ID: wpr-233628
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.</p><p><b>METHODS</b>HSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.</p><p><b>RESULTS</b>Compared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.</p><p><b>CONCLUSION</b>IL-10 inhibits activation of HSC through the PDGF/MAPK pathway.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Platelet-Derived Growth Factor / Signal Transduction / Cell Line / Interleukin-10 / Mitogen-Activated Protein Kinases / Hepatocytes / Cell Biology / Cell Proliferation Limits: Animals Language: Chinese Journal: Chinese Journal of Hepatology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Platelet-Derived Growth Factor / Signal Transduction / Cell Line / Interleukin-10 / Mitogen-Activated Protein Kinases / Hepatocytes / Cell Biology / Cell Proliferation Limits: Animals Language: Chinese Journal: Chinese Journal of Hepatology Year: 2005 Type: Article