Detection of miR-122a and miR-224 expression in hepatocellular carcinoma by real-time fluorescence quantitative RT-PCR / 南方医科大学学报
Journal of Southern Medical University
;
(12): 751-753, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233692
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expressions of miR-122 and miR-224 in hepatocellular carcinoma (HCC) by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC.</p><p><b>METHOD</b>2(-Delta Delta CT) method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting.</p><p><b>RESULTS</b>Compared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation (P<0.01) and miR-224 over-expression (P<0.001).</p><p><b>CONCLUSIONS</b>HCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spectrometry, Fluorescence
/
Time Factors
/
Base Sequence
/
Gene Expression Regulation, Neoplastic
/
Blotting, Northern
/
Carcinoma, Hepatocellular
/
Reverse Transcriptase Polymerase Chain Reaction
/
MicroRNAs
/
Diagnosis
/
Genetics
Type of study:
Diagnostic study
/
Screening study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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