Recombinant adenovirus-mediated expression of 1.2-copy hepatitis B virus DNA in three hepatocytes / 南方医科大学学报
Journal of Southern Medical University
;
(12): 738-740, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233695
ABSTRACT
<p><b>OBJECTIVE</b>To study hepatitis B virus (HBV) expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA.a</p><p><b>METHODS</b>A chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection.</p><p><b>RESULTS</b>HBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time.a</p><p><b>CONCLUSION</b>Infection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
DNA, Recombinant
/
DNA, Viral
/
Gene Expression
/
Adenoviridae
/
Hepatitis B virus
/
Culture Media, Conditioned
/
Reverse Transcriptase Polymerase Chain Reaction
/
Hepatocytes
/
Cell Line, Tumor
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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