Effect of angiotensin1-7 on alpha-smooth muscle actin protein expression in rat hepatic stellate cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 732-733, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233697
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of angiotensin II and angiotensin1-7 on alpha-smooth muscle actin (alpha-SMA)-induced Ca(2+)-independent pathways mediated by Rho kinase2 in hepatic stellate cells (HSCs).</p><p><b>METHODS</b>HSC-T6 cells were treated with 10 micromol/L of AngII, Ang1-7, AngII +Ang1-7, and Ang1-7+A779. RT-PCR was used to detect the expression of Rho kinase2 (Rock2) in Ca(2+)-independent pathways, and alpha-SMA protein expression was detected by Western blotting.</p><p><b>RESULTS</b>The mRNA expression of Rock2 increased significantly in the cells after AngII treatment (P<0.01), but decreased following Ang1-7 treatment. Ang1-7 treatment significantly reduced alpha-SMA level in AngII-induced cells (P<0.01).</p><p><b>CONCLUSION</b>Ang1-7 can inhibit AngII-induced activation of Rock2 and reduce alpha-SMA expression in HSCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Pharmacology
/
RNA, Messenger
/
Angiotensin I
/
Angiotensin II
/
Signal Transduction
/
Cell Line
/
Gene Expression Regulation
/
Calcium
/
Actins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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