Effect of Shuanghuanglian injection on cerebral nuclear factor-kappaB expression in mice with viral encephalitis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 676-679, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233712
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Shuanghuanglian injection on cerebral expression of nuclear factor-kappaB (NF-kappaB) in mice with viral encephalitis.</p><p><b>METHODS</b>The mice with experimental viral encephalitis received treatment with Shuanghuanglian injection at the dose of 0.2, 1.5, and 5 for 5, 10 or 20 consecutive days. The total RNA of the brain tissue was extracted to analyze the protein and mRNA expression of NF-kappaB using Western blotting and RT-PCR, respectively.</p><p><b>RESULTS</b>Compared with the control group, the mice with experimental viral encephalitis showed significantly increased protein and mRNA expressions of NF-kappaB (P<0.01). Treatment with Shuanghuanglian injection at the doses of 0.2 and 1.5 mg/kg significantly lowered NF-kappaB protein and mRNA expressions in the brain of mice with viral encephalitis (P<0.05), and the effect was even more obvious at the dose of 5 mg/kg (P<0.01).</p><p><b>CONCLUSION</b>Shuanghuanglian injection can reduce the expression of NF-kappaB in the brain of mice with viral encephalitis in a dose- and time-dependent manner.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Brain
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Gene Expression Regulation
/
Blotting, Western
/
NF-kappa B
/
Encephalitis, Viral
/
Reverse Transcriptase Polymerase Chain Reaction
/
Therapeutic Uses
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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