Establishment and assessment of Caco-2 cell in vitro absorption model / 南方医科大学学报
Journal of Southern Medical University
;
(12): 548-550, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233737
ABSTRACT
<p><b>OBJECTIVE</b>To establish and assess the Caco-2 cell in vitro absorption model.</p><p><b>METHODS</b>Caco-2 cells were cultured on the millipore filters fixed in Snapwell transport chamber. The cell morphology, transepithelial electrical resistance, mannitol efflux rate and alkaline phosphatase activities were monitored during culture.</p><p><b>RESULTS</b>After 21 days of in vitro culture, formation of tight junction was observed between the cells. The transepithelial electrical resistance reach a relatively stable value of 620-/+47 Omega.cm(2), the mannitol efflux rate was lower than 0.3%.h(-1).cm(-2), and the alkaline phosphatase activity in the apical side was significantly higher than that in the basolateral side.</p><p><b>CONCLUSION</b>The established Caco-2 cell model shows similar morphology to intestinal epithelial cells with formation of polarity, and can be used as an in vitro model for absorption studies.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Culture Techniques
/
Caco-2 Cells
/
Cell Biology
/
Epithelial Cells
/
Intestinal Absorption
/
Intestines
/
Metabolism
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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