Effects of celecoxib on PGE2 synthesis and COX-2 and VEGF-C mRNA expression in Tca8113 cell lines / 南方医科大学学报
Journal of Southern Medical University
;
(12): 466-468, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233762
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on prostaglandin E(2) (PGE2) release and vascular endothelial growth factor C (VEGF-C) and COX-2 mRNA expression in Tca8113 cell lines.</p><p><b>METHODS</b>MTT assay was used to analyze the proliferation of Tca8113 cells. The PGE2 level was detected with enzyme-linked immunosorbent assay (ELISA), and the expressions of COX-2 and VEGF-C mRNA were examined with RT-PCR.</p><p><b>RESULTS</b>Celecoxib could induce inhibitory effects on the growth and PGE2 release in Tca8113 cells. The RT-PCR results showed that celecoxib significantly down-regulated the expression of VEGF-C mRNA, but produced a weak effect on COX-2 mRNA expression.</p><p><b>CONCLUSION</b>The inhibitory effect of celecoxib on Tca8113 cell growth and the expressions of VEGF-C and COX-2 may be related to the release of PGE2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Pyrazoles
/
Sulfonamides
/
RNA, Messenger
/
Tongue Neoplasms
/
Carcinoma, Squamous Cell
/
Dinoprostone
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor C
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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