p35 and p25 expressions and Cdk5 kinase activity in primary cultured rat hippocampal neurons with X-ray exposure / 南方医科大学学报
Journal of Southern Medical University
;
(12): 405-407, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-233777
ABSTRACT
<p><b>OBJECTIVE</b>To study the expressions of p35 and p25 and Cdk5 kinase activity in cultured rats hippocampal neurons following X-ray exposure to provide experimental evidence for prevention and treatment of radiation encephalopathy.</p><p><b>METHODS</b>The hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy. Western blotting was used to detect the p35 and p25 protein levels, and the effect of pretreatment with roscovitine, a Cdk5 inhibitor, on the apoptosis of the hippocampal neurons following the exposure was examined with 4',6-diamidino-2-phenylindole (DAPI) staining.</p><p><b>RESULTS</b>The protein level of p35 increased significantly 3.5 and 4 h after the irradiation by 1.51-/+0.13 and 1.45-/+0.14 folds in comparison with the control level, respectively (P<0.01), and the p25 level increased significantly 6 h after irradiation by 1.62-/+0.28 folds (P<0.05). Nuclear condensation occurred in (24.8-/+3.97)% of the neurons 24 h after 30 Gy X-ray exposure, a rate significantly higher than that in the nonexposed cells [(1.82-/+1.08)%, P<0.01) and that in roscovitine-pretreated neurons [(7.74-/+2.27)%, P<0.01).</p><p><b>CONCLUSION</b>X-ray exposure activates Cdk5 by increasing the p35 and p25 expressions in rat hippocampal neurons, and inhibition of Cdk5 activity with roscovitine can significantly protect the neurons from apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Radiation Effects
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Cell Biology
/
Cyclin-Dependent Kinase 5
/
Genetics
/
Hippocampus
/
Animals, Newborn
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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