Inhibition of nuclear translocation and binding activity of nuclear factor-kappaB by oligodeoxynucleotides in THP-1 cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 102-104, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-234185
ABSTRACT
<p><b>OBJECTIVE</b>To observe the inhibitory effect of oligodeoxynucleotides (ODN) on nuclear translocation and nuclear binding activity of nuclear factor (NF)-kappaB in THP-1 cells.</p><p><b>METHODS</b>Oligodeoxynucleotides were transfected via liposome into THP-1 cells followed by stimulation of the cells with lipopolysaccharide (LPS). Immunocytochemistry, electrophoretic mobility shift assay (EMSA) and reverse transcription (RT)-PCR were performed to detect the nuclear translocation and nuclear binding activity of NF-kappaB.</p><p><b>RESULTS</b>Immunocytochemical results showed that after LPS stimulation of the ODN-transfected cells, NF-kappaB expression was still localized in cytoplasma. EMSA demonstrated inhibited nuclear binding activity of NF-kappaB in the ODN-transfected cells, and ODN inhibited the mRNA expression of tumor necrosis factor (TNF)-alpha, a NF-kappaB-associated inflammatory factor, as shown by RT-PCR.</p><p><b>CONCLUSION</b>ODN can inhibit the nuclear translocation and binding activity of NF-kappaB in THP-1 cells, whereby the transcription and expression of the related inflammation factor genes is suppressed, which shed light on a new solution for clinical treatment of acute pancreatitis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligodeoxyribonucleotides
/
Pharmacology
/
Transcription Factors
/
Transfection
/
Cell Line
/
Lipopolysaccharides
/
NF-kappa B
/
Cell Biology
/
Macrophages
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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