Arsenic trioxide induces socs-1 gene demethylation in myeloma cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1064-1068, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-234299
ABSTRACT
The aim of this study was to explore the effect of arsenic trioxide (As(2)O(3)) on the methylation status of socs-1 gene in multiple myeloma cell lines U266, RPMI8226. The cell viability was assayed by MTT method. The methylation status of socs-1 gene was detected by methylation specific PCR. The expression of socs-1 gene mRNA was determined with real-time PCR. The cell apoptosis was analyzed by flow cytometry. The results indicated that hypermethylation of CpG island of socs-1 gene was observed without expression of socs-1 in myeloma cell lines U266, RPMI8226. The expression of socs-1 gene mRNA in each myeloma cell line increased significantly after exposure to As(2)O(3) for 72 hours as compared with the cell lines of wild type (p < 0.05). And cell proliferation was significantly inhibited, both early apoptosis and later apoptosis ratios increased in dose-dependent manner. It is concluded that As(2)O(3) may induce socs-1 demethylation and up-regulate the expression of the gene. This study provides a new thought and direction for exploring possible mechanism of cell apoptosis induced by As(2)O(3) and multiple myeloma treatment by As(2)O(3).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxides
/
Pharmacology
/
Arsenicals
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
CpG Islands
/
DNA Methylation
/
Cell Line, Tumor
/
Suppressor of Cytokine Signaling Proteins
/
Suppressor of Cytokine Signaling 1 Protein
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
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