Effect of siRNA targeting PML-RARa fusion gene on activity of the acute promyelocytic leukemia cell line NB4 / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 850-854, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-234497
ABSTRACT
This study aims to investigate the effects of small interference RNA (siRNA) targeting PML-RARa mRNA on the activity of the acute promyelocytic leukemia cell line NB4. The proliferation inhibition was evaluated by MTT assay and colony-formation inhibiting test. Apoptosis was determined by flow cytometry after siRNA treatment. The results showed that the cell growth of siRNA treated group was inhibited, and the apoptosis of NB4 could be induced. The siRNA targeting PML-RARα mRNA might be a valid therapy of acute promyelocytic leukemia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Leukemia, Promyelocytic, Acute
/
Oncogene Proteins, Fusion
/
Cell Cycle
/
Apoptosis
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2014
Type:
Article
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