Ectopic osteogenesis in vivo using bone morphogenetic protein-2 derived peptide loaded biodegradable hydrogel / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 811-815, 2014.
Article
in Zh
| WPRIM
| ID: wpr-234505
Responsible library:
WPRO
ABSTRACT
We investigated the development of an injectable, biodegradable hydrogel composite of poly(trimethylene carbonate)-F127-poly(trimethylene carbonate)(PTMC11-F127-PTMC11 )loaded with bone morphogenetic protein-2 (BMP-2) derived peptide P24 for ectopic bone formation in vivo and evaluated its release kinetics in vitro. Then we evaluated P24 peptide release kinetics from different concentration of PTMC11-F127-PTMC11 hydrogel in vitro using bicinchoninic acid (BCA)assay. P24/ PTMC11-F127-PTMC11 hydrogel was implanted into each rat's erector muscle of spine and ectopic bone formation of the implanted gel in vivo was detected by hematoxylin and eosin stain (HE). PTMC11-F127-PTMC11 hydrogel with concentration more than 20 percent showed sustained slow release for one month after the initial burst release. Bone trabeculae surround the P24/ PTMC11-F127-PTMC11 hydrogel was shown at the end of six weeks by hematoxylin and eosin stain. These results indicated that encapsulated bone morphogenetic protein (BMP-2) derived peptide P24 remained viable in vivo, thus suggesting the potential of PTMC11-F127-PT- MC11 composite hydrogels as part of a novel strategy for localized delivery of bioactive molecules.
Full text:
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Index:
WPRIM
Main subject:
Osteogenesis
/
Peptides
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Pharmacology
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Prostheses and Implants
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Biocompatible Materials
/
Bone and Bones
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Chemistry
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Drug Delivery Systems
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Bone Morphogenetic Proteins
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Hydrogels
Limits:
Animals
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2014
Type:
Article