Effects of bears' bile on the expression of tumor cell p53 protein / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 86-88, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-234800
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of bears' bile on tumor cell p53 protein expression with different gene properties.</p><p><b>METHODS</b>The effects of bears' bile on the expression of p53 protein in 6 cancer cell strains were determined by Western blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Results Western blot analysis showed that the expression of p53 protein in HaCaT, KUMA3, KUMA4 and KUMA6 cell strains with gene mutation were increased, but no change was found in HCT116 and KUMA5 cell strains without gene mutation. There was no quantitative change in p53 mRNA in all cell strains by analysis of p53 mRNA with</p><p><b>CONCLUSION</b>The effects of bears' bile on p53 protein expression in cancer cell strains RT-PCR analysis system. could be different based on p53 gene properties,i. e. ,bears' bile only affect p53 protein of mutation type.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Ursidae
/
Bile
/
RNA, Messenger
/
Biological Factors
/
Tumor Suppressor Protein p53
/
Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2006
Type:
Article
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