Effect of curcumin on expression of human low density lipoprotein receptors in Xenopus Laevis oocytes / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 432-435, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-234855
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanism of curcumin in reducing blood lipids by establishing gene expression system of human low density lipoprotein receptors (LDL-R) in Xenopus Laevis oocytes (XLO).</p><p><b>METHODS</b>The expression of LDL-R on cytomembrane was determined using immuno-fluorescent, ligand-fluorescent and immune colloidal gold techniques after human LDL-R containing p3.7 LDL plasmid was led into nucleus. And the expression of LDL-R gene in XLO was quantitatively determined by ELISA after being interfered with different concentrations of curcumin.</p><p><b>RESULTS</b>The human LDL-R gene could be expressed on XLO, which could be significantly enhanced by curcumin in a dose-dependent manner. Conclusion One of the paths of curcumin in reducing blood lipids and anti-atherosclerosis was improving LDL-R gene expression and increasing the LDL-cholesterol absorption of cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Pharmacology
/
Xenopus laevis
/
Receptors, LDL
/
Cells, Cultured
/
Gene Expression Regulation
/
Curcumin
/
Cell Biology
/
Dose-Response Relationship, Drug
/
Genetics
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2005
Type:
Article
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