Effect of Bushen recipe on T-cell subsets and their function in patients with chronic hepatitis B / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 408-411, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-234862
ABSTRACT
<p><b>OBJECTIVE</b>To observe the regulatory effect of Bushen recipe (BSR) on immune function in patients with chronic hepatitis B.</p><p><b>METHODS</b>Thirty patients with HBeAg+ and abnormal alanine transaminase (ALT) level were treated with BSR for 6 months. The following parameters before treatment, 3 months and 6 months after treatment were tested (1) HBV DNA by fluorescence quanitiative PCR; (2) Levels of interleukin-10 (IL-10) and interferon-gamma in supernatant of cultured peripheral blood mononuclear cell (PBMC) after had been cultured with HBeAg, HBcAg and phytohemagglutinin (PHA) for 48 hrs; (3) Blood levels of T-cells, including CD4+ , CD8+ , CD8+ CD28+ , CD8+ CD28- and CD28+ , and their ratio, as well as the percentage of CD8+ CD28+ in the cell culture after being co-cultured with PHA for 48 hrs.</p><p><b>RESULTS</b>In patients effectively treated by BSR, after 3 months treatment, level of IFN-gamma in PBMC culture significantly increased, CD8+ CD28+ , CD28+ T-cells significantly enhanced and IL-10 and CD8 + CD28+ T-cells significantly decreased (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>BSR could significantly strengthen the expression of Th1 type cytokine, decrease the expression of Th2 type cytokine and promote the expression of CD8+ cytotoxic T-lymphocyte, which may be one of mechanisms in suppressing HBV DNA replication.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virus Replication
/
Blood
/
Drugs, Chinese Herbal
/
Hepatitis B virus
/
T-Lymphocyte Subsets
/
Interleukin-10
/
Th2 Cells
/
Th1 Cells
/
Hepatitis B, Chronic
/
Therapeutic Uses
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2005
Type:
Article
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