Experimental study of adipose-derived mesenchymal stem cells in the treatment of Crohn's disease / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 58-64, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-234958
ABSTRACT
<p><b>OBJECTIVE</b>To explore the efficacy of adipose-derived mesenchymal stem cells (ADMSCs) in a murine model of inflammatory bowel disease, and its potential mechanism.</p><p><b>METHODS</b>Murine colitis mouse model of Crohn's disease(CD) was created by trinitrobenzene sulfonic acid(TNBS)-induced colitis. Seventy-five 6-8 weeks female BALB/c mice were randomly divided into 3 groups control group, TNBS group and ADMSC group. To verify the therapeutic effect of ADMSC, real-time PCR and immunohistochemical staining were performed to measure inflammatory cytokines levels in colon tissues. The 10-day survival statuses were recorded after the infusion of ADMSCs.</p><p><b>RESULTS</b>Intraperitoneal injection of ADMSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and increased survival(60% vs. 30%, P<0.05) in the TNBS-induced mouse model of CD. Compared with TNBS group, proinflammatory cytokines, including TNF-α, IL-12 and VEGF of ADMSC group were significantly reduced, with significant increase of IL-10 expression.</p><p><b>CONCLUSION</b>ADMSCs can effectively repair the injury of colonitis through down-regulation of proinflammatory cytokines TNF-α, IL-12 and VEGF expression, and up-regulation of anti-inflammatory cytokine IL-10 expression, which may be a potential new alternative of cell-based therapy for CD.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trinitrobenzenesulfonic Acid
/
Inflammatory Bowel Diseases
/
Crohn Disease
/
Down-Regulation
/
Up-Regulation
/
Cytokines
/
Colitis
/
Adipocytes
/
Disease Models, Animal
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2015
Type:
Article
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