An animal experiment of construction of functional tissue-engineered bone with cell sheet technology / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering.</p><p><b>METHODS</b>Canine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 degrees C, 5% CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 degrees C. Demineralized bone matrix (DBM) and platelet-rich plasma (PRP) were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle.</p><p><b>RESULTS</b>When temperature dropped at 20 degrees C, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group.</p><p><b>CONCLUSION</b>Cell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone.</p>