A comparison of the myogenic characteristics between stem cell antigen-(1+) and stem cell antigen-(1-) cells derived from skeletal muscle / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To probe into the function of stem cell antigen-1 (Sca-1) in cell proliferation and differentiation.</p><p><b>METHODS</b>Sca-(1+) and Sca-(1-) muscle derived cells were separated from C57BU6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca(-1), MyoD and Myogenin expression in cultured Sca(-1+) and Sca-(1-) muscle derived cells.</p><p><b>RESULTS</b>The difference of the proliferative curve of Sca-(1+) and Sca-(1-) muscle derived cells cultured 3 days in vitro was not apparent, but Sca-(1-) muscle derived cells had a accelerated division rate in the follow days compared to the Sca-(1+) muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-(1+) than Sca-(1-) muscle derived cells.</p><p><b>CONCLUSION</b>Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.</p>