On-line detection of hydrocortisone concentration in rat subcutaneous adipose with liquid chromatography-mass spectrometry/mass spectrometry / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1387-1391, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-235117
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for detecting the topical concentration of hydrocortisone (HC) in the subcutaneous adipose of rats using microdialysis sampling technique and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS).</p><p><b>METHODS</b>Topical samples were collected by applying the probe into the subcutaneous adipose of rats, with alcohol (5%)-ringers solution as the perfusion solution. A LC-MS/MS method was established for detecting the HC concentration in the dialysates.</p><p><b>RESULTS</b>The protonated precursor to produce ion transitions monitored for HC was m/z 363.2→121.1. The calibration curve was linear over the range of 0.5-1000 ng/ml, with the intra- and inter-day precision and accuracy within ∓15%, and no significant matrix effect was noted. The in vivo recovery of the probe was about 59%.</p><p><b>CONCLUSION</b>A selective and sensitive method has been successfully established for the on-line HC detection in the subcutaneous adipose of rats.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Administration, Cutaneous
/
Hydrocortisone
/
Pharmacokinetics
/
Chemistry
/
Chromatography, Liquid
/
Rats, Wistar
/
Microdialysis
/
Subcutaneous Fat
/
Tandem Mass Spectrometry
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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