Effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on human gastric cancer cells and its mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1345-1348, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-235127
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis-inducing effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), a compound isolated from Pteris semipinnata L (PsL), on human gastric cancer SGC7901 cells and explore its mechanism.</p><p><b>METHODS</b>The inhibitory effect of 5F on SGC7901 cells was observed by MTT assay and flow cytometry, and the changes of the expression of Bcl-2 and Bax in SGC7901 cells following 5F exposure were evaluated by Western blot analysis.</p><p><b>RESULTS</b>5F inhibited the proliferation of SGC7901 cells in a concentration- and time-dependent manner, and the cell apoptosis induced by 5F was confirmed by Annexin V-EGFP staining and caspase-3 activation assay. The cell apoptosis induced by 5F was associated with decreased Bcl-2 and increased Bax expressions.</p><p><b>CONCLUSION</b>5F exposure induces apoptosis in SGC7901 cells by activating mitochondrial apoptotic pathways.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Stomach Neoplasms
/
Chemistry
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Pteris
/
Cell Line, Tumor
/
Cell Proliferation
/
Diterpenes
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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