Feasibility of peptide mass fingerprinting for differential diagnosis of IgA and non-IgA nephropathy / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1309-1313, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-235136
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of peptide mass fingerprinting for non-invasive differential diagnosis of IgA nephropathy (IgAN) from the non-IgA nephropathy (IgAN).?</p><p><b>METHODS</b>According to the results of renal biopsy, 56 patients were divided into IgAN group (n=28) and non-IgAN group (n=28), and peptide mass fingerprints were acquired from these patients using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).</p><p><b>RESULTS</b>Nine different peptides were identified between IgAN and non-IgAN. The two most distinctive differentially expressed peptides, with peptide peak values of 4476.46 and 1968.10, showed area under curve values of 86.18% and 79.77%. Principal component analysis demonstrated that the accumulated explained variance of the first 8 differential peptides reached 95%, suggesting the feasibility of differential diagnosis of IgAN from non-IgAN. Comparison with the Matrix protein database identified the peptide with a relative molecular mass of 5338.08 as a fragment of mucin 4 inform and the 2082.77 peptide as fragment of α1-II type collagen inform.</p><p><b>CONCLUSION</b>MALDI-TOF MS is feasible for differential diagnosis of IgAN and non-IgAN and also has great potentials in the classification of the subtypes of other systemic diseases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Peptide Mapping
/
Chemistry
/
Feasibility Studies
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Diagnosis
/
Diagnosis, Differential
/
Glomerulonephritis, IGA
/
Kidney Diseases
/
Methods
Type of study:
Diagnostic study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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