Mechanism of E1A-mediated escape from ras-induced senescence in human fibraoblasts / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1392-1395, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-235141
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of binding activities of the NH(2) terminus of E1A to the proteins regulating cell growth on ras-induced cell senescence and explore the mechanism of E1A-mediated escape from ras-induced senescence by E1A in human fibroblast.</p><p><b>METHODS</b>In primary human fibroblasts, the proteins regulating cell growth in association with E1A NH(2) terminus, including the Rb family proteins, p300/CBP, and p400, were inactivated or interfered. The effect of alterations in the binding activities of these proteins on cell senescence bypass mediated by E1A was evaluated by cell growth curve.</p><p><b>RESULTS</b>The Inactivation of Rb family proteins alone was not sufficient to rescue ras-induced cell senescence, whereas inactivation of both the Rb proteins and p300/CBP blocked ras-induced senescence of human fibroblasts.</p><p><b>CONCLUSION</b>Rb and p300/CBP binding activities are both required for E1A to bypass ras-induced senescence in human fibroblasts.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Skin
/
Retinoblastoma Protein
/
Cellular Senescence
/
Adenovirus E1A Proteins
/
Ras Proteins
/
Cell Biology
/
P300-CBP Transcription Factors
/
Primary Cell Culture
/
Fibroblasts
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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