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Mechanism of E1A-mediated escape from ras-induced senescence in human fibraoblasts / 南方医科大学学报
Journal of Southern Medical University ; (12): 1392-1395, 2011.
Article in Chinese | WPRIM | ID: wpr-235141
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of binding activities of the NH(2) terminus of E1A to the proteins regulating cell growth on ras-induced cell senescence and explore the mechanism of E1A-mediated escape from ras-induced senescence by E1A in human fibroblast.</p><p><b>METHODS</b>In primary human fibroblasts, the proteins regulating cell growth in association with E1A NH(2) terminus, including the Rb family proteins, p300/CBP, and p400, were inactivated or interfered. The effect of alterations in the binding activities of these proteins on cell senescence bypass mediated by E1A was evaluated by cell growth curve.</p><p><b>RESULTS</b>The Inactivation of Rb family proteins alone was not sufficient to rescue ras-induced cell senescence, whereas inactivation of both the Rb proteins and p300/CBP blocked ras-induced senescence of human fibroblasts.</p><p><b>CONCLUSION</b>Rb and p300/CBP binding activities are both required for E1A to bypass ras-induced senescence in human fibroblasts.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Skin / Retinoblastoma Protein / Cellular Senescence / Adenovirus E1A Proteins / Ras Proteins / Cell Biology / P300-CBP Transcription Factors / Primary Cell Culture / Fibroblasts Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Skin / Retinoblastoma Protein / Cellular Senescence / Adenovirus E1A Proteins / Ras Proteins / Cell Biology / P300-CBP Transcription Factors / Primary Cell Culture / Fibroblasts Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2011 Type: Article