Neuroprotective Effect of Rapamycin in Optic Nerve Transection Model
Journal of the Korean Ophthalmological Society
;
: 1150-1156, 2012.
Article
in Korean
| WPRIM
| ID: wpr-23521
ABSTRACT
PURPOSE:
The present study investigated whether rapamycin activated autophagy in retinal ganglion cells (RGC) and evaluated its effect on RGC survival following optic nerve transection (ONT).METHODS:
The activation of autophagy in RGCs after intravitreal injection of rapamycin was evaluated with the immunohistochemical staining of phospho-S6 ribosomal protein. Rapamycin or 0.1% DMSO was injected intravitreally immediately after ONT. At 1 and 2 weeks after ONT, the RGCs were counted. Rapamycin and autophagy inhibitors, 3-methyladenine or Wortmannin were co-injected intravitreally after ONT and the RGCs were counted 1 week later.RESULTS:
Expression of phospho-S6 ribosomal protein was decreased in RGCs after intravitreal injection of rapamycin. The RGC number was significantly higher in the rapamycin group than in the control group 1 week after ONT. However, the RGC number was not different between the 2 groups 2 weeks after ONT. Repeated intravitreal injection of rapamycin at 1-week intervals showed neuroprotection 2 weeks after ONT. The RGC number was not different between the control group and the co-injection group of rapamycin-autophagy inhibitor.CONCLUSIONS:
Activated autophagy by rapamycin was neuroprotective in RGC after ONT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Optic Nerve
/
Retinal Ganglion Cells
/
Ribosomal Proteins
/
Autophagy
/
Adenine
/
Dimethyl Sulfoxide
/
Neuroprotective Agents
/
Sirolimus
/
Optic Nerve Injuries
/
Intravitreal Injections
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2012
Type:
Article
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