Determination of three glycosides from herbs of Swertia punicea by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for determination of three glycosides in Swertia punicea.</p><p><b>METHOD</b>Chromatographic column Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate 1 mL x min(-1). Wavelength 254 nm. Column temperture 30 degrees C.</p><p><b>RESULT</b>The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).</p><p><b>CONCLUSION</b>It is a simple and sensitive meathod in controlling the quality of S. punicea.</p>