Regulation of E-cadherin promoter activity by LMO2 impact on the progression and metastasis of prostate cancer / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 385-389, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-235352
ABSTRACT
<p><b>OBJECTIVE</b>To study the abnormal expression of the proto-oncogene LMO2 affect the progression and metastasis mechanism of prostate cancer.</p><p><b>METHODS</b>A series of reporter gene expression vectors carrying different lengths and point mutations of E-cadherin promoter were constructed. These plasmids were separately co-transfected with LMO2 into Lncap cells and the luciferase activity was detected after 24 h.</p><p><b>RESULTS</b>The overexpression of LMO2 could significantly inhibit the activity of luciferase reporter gene of E-cadherin promoter about 50%. Truncated and point mutation study showed that this was mainly through E-box sites in the promoter region -204/-198.</p><p><b>CONCLUSION</b>The proto-oncogene LMO2 can affect the progression and metastasis mechanism of prostate cancer by transcriptional inhibition of E-cadherin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Prostatic Neoplasms
/
Cadherins
/
Proto-Oncogene Proteins
/
Promoter Regions, Genetic
/
Point Mutation
/
Cell Line, Tumor
/
Adaptor Proteins, Signal Transducing
/
LIM Domain Proteins
/
Genetic Vectors
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS