Effects of endothelial progenitor cell-conditioned medium on the proliferation, adhesion and migration of vascular smooth muscle cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 199-202, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-235402
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of endothelia progenitor cells conditioned medium (EPC-CM) on the migration, adhesion and proliferation of vascular smooth muscle cells (VSMCs).</p><p><b>METHODS</b>Mononuclear cells were isolated from rat bone marrow by density gradient centrifugation,plated on dishes precoated with 5% fibronectin, and then cultured with complete M199 medium (including 15% fetal calf serum, 10 microg/L VEGF and 5 microg/L bFGF). EPC-CM was collected and used to incubate VSMCs isolated from rat arteriae aorta. After 24 h, VSMCs proliferation, adhesion and migration were assayed with CCK-8, adhesion test and modified Boyden chamber assay, respectively.</p><p><b>RESULTS</b>The proliferation, adhesion and migration of VSMCs were obviously decreased when the cells were cultured with EPC-CM.</p><p><b>CONCLUSION</b>EPC-CM could inhibit VSMC functions, which would be one of the mechanisms against atherosclerosis by EPCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Cell Adhesion
/
Cell Movement
/
Cells, Cultured
/
Chemistry
/
Rats, Sprague-Dawley
/
Culture Media, Conditioned
/
Cell Biology
/
Endothelial Cells
/
Cell Proliferation
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2013
Type:
Article
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