Your browser doesn't support javascript.
loading
Prediction of ocular irritancy of 26 chemicals and 26 cosmetic products with isolated rabbit eye (IRE) test / 生物医学与环境科学(英文)
Article in En | WPRIM | ID: wpr-235547
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.</p><p><b>METHODS</b>IRE test was performed according to modifications of the in vitro toxicology (INVITTOX) Protocol No.85: Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM), and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.</p><p><b>RESULTS</b>IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test χ(2)=51.314, P<0.001; McNemar P=0.261; Gamma=0.960, P<0.001; Kappa=0.843, P<0.001) and 26 cosmetic products (Fisher's Exact Test χ(2)=15.522, P<0.001; McNemar P=0.311; Gamma=0.967, P<0.001; Kappa=0.611, P<0.001).</p><p><b>CONCLUSION</b>IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Toxicity Tests / Cosmetics / Toxicity / Eye / Irritants / Animal Testing Alternatives / Methods Type of study: Prognostic_studies Limits: Animals Language: En Journal: Biomedical and Environmental Sciences Year: 2012 Type: Article
Full text: 1 Index: WPRIM Main subject: Toxicity Tests / Cosmetics / Toxicity / Eye / Irritants / Animal Testing Alternatives / Methods Type of study: Prognostic_studies Limits: Animals Language: En Journal: Biomedical and Environmental Sciences Year: 2012 Type: Article