Echinococcus granulosus 14-3-3 protein: a potential vaccine candidate against challenge with Echinococcus granulosus in mice / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 352-358, 2012.
Article
in English
| WPRIM
| ID: wpr-235548
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.</p><p><b>METHODS</b>ICR mice were subcutaneously immunized three times with rEg14-3-3, followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay, and to measure the secretion of IL-2, IL-4, IL-10, and IFN-γ by ELISA. The rate of reduced hydatid cyst and the levels of IgE, IgG and IgG subclasses in sera were examined.</p><p><b>RESULTS</b>Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47%. ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a. Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response. The secretion of IFN-γ and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.</p><p><b>CONCLUSION</b>The results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Spleen
/
Enzyme-Linked Immunosorbent Assay
/
Vaccines
/
Gene Expression Regulation
/
Blotting, Western
/
Cytokines
/
Cell Biology
/
Echinococcus granulosus
/
14-3-3 Proteins
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2012
Type:
Article
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