Determination of taurine in biological samples by high-performance liquid chromatography using 4-fluoro-7-nitrobenzofurazan as a derivatizing agent / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 537-542, 2011.
Article
in English
| WPRIM
| ID: wpr-235599
ABSTRACT
<p><b>OBJECTIVE</b>A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples, including plasma, brain, and liver.</p><p><b>METHODS</b>The optimum derivatization reaction temperature was 70 °C, and at this temperature the reaction was complete within 3 min. The derivatized taurine was separated using phosphate buffer (0.02 mol/L, pH 6.0)acetonitrile (8416, v/v) as the mobile phase at a flow rate of 1.0 mL/min, and a column temperature of 25 °C. The taurine derivatives were separated within 20 min (tR14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.</p><p><b>RESULTS</b>The intra- and the inter-day coefficients of variation for the method were 5.3% and 7.7%, respectively. The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.</p><p><b>CONCLUSION</b>This method can be used to determine the taurine contents in plasma, brain, and liver from normal rats and human plasma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetonitriles
/
Solvents
/
Taurine
/
Temperature
/
Blood
/
Brain Chemistry
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Rats, Wistar
/
Fluorescent Dyes
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2011
Type:
Article
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